Initial recommendations for performing, benchmarking and reporting single-cell proteomics experiments.
Nat Methods
; 20(3): 375-386, 2023 03.
Article
em En
| MEDLINE
| ID: mdl-36864200
ABSTRACT
Analyzing proteins from single cells by tandem mass spectrometry (MS) has recently become technically feasible. While such analysis has the potential to accurately quantify thousands of proteins across thousands of single cells, the accuracy and reproducibility of the results may be undermined by numerous factors affecting experimental design, sample preparation, data acquisition and data analysis. We expect that broadly accepted community guidelines and standardized metrics will enhance rigor, data quality and alignment between laboratories. Here we propose best practices, quality controls and data-reporting recommendations to assist in the broad adoption of reliable quantitative workflows for single-cell proteomics. Resources and discussion forums are available at https//single-cell.net/guidelines .
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Benchmarking
/
Proteômica
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article