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Slc9a1 plays a vital role in chitosan oligosaccharide transport across the intestinal mucosa of mice.
Wen, Jiaying; Chen, Shengwei; Bao, Minglong; Hu, Canying; Wu, Lianyun; Yong, Yanhong; Liu, Xiaoxi; Li, Youquan; Yu, Zhichao; Ma, Xingbin; Eun, Jong-Bang; Shim, Jae-Han; Warda, Mohamad; Abd El-Aty, A M; Ju, Xianghong.
Afiliação
  • Wen J; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Pr
  • Chen S; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Pr
  • Bao M; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Pr
  • Hu C; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • Wu L; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China.
  • Yong Y; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Pr
  • Liu X; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
  • Li Y; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
  • Yu Z; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
  • Ma X; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Province, Zhanjiang 524088, China.
  • Eun JB; Department of Food Science and Technology, Chonnam National University, Gwangju, Republic of Korea.
  • Shim JH; Natural Products Chemistry Laboratory, Biotechnology Research Institute, Chonnam National University, Yongbong-ro, Buk-gu, Gwangju 500-757, Republic of Korea.
  • Warda M; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
  • Abd El-Aty AM; State Key Laboratory of Biobased Material and Green Papermaking, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical Pharmacology, Medical Faculty, Ata
  • Ju X; Marine Medical Research and Development Centre, Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China; The Key Laboratory of Animal Resources and Breed Innovation in Western Guangdong Pr
Carbohydr Polym ; 299: 120179, 2023 Jan 01.
Article em En | MEDLINE | ID: mdl-36876794
ABSTRACT
The mechanism underlying the intestinal transport of COS is not well understood. Here, transcriptome and proteome analyses were performed to identify potential critical molecules involved in COS transport. Enrichment analyses revealed that the differentially expressed genes in the duodenum of the COS-treated mice were mainly enriched in transmembrane and immune function. In particular, B2 m, Itgb2, and Slc9a1 were upregulated. The Slc9a1 inhibitor decreased the transport efficiency of COS both in MODE-K cells (in vitro) and in mice (in vivo). The transport of FITC-COS in Slc9a1-overexpressing MODE-K cells was significantly higher than that in empty vector-transfected cells (P < 0.01). Molecular docking analysis revealed the possibility of stable binding between COS and Slc9a1 through hydrogen bonding. This finding indicates that Slc9a1 plays a crucial role in COS transport in mice. This provides valuable insights for improving the absorption efficiency of COS as a drug adjuvant.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transporte Biológico / Quitosana / Trocador 1 de Sódio-Hidrogênio / Mucosa Intestinal Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Transporte Biológico / Quitosana / Trocador 1 de Sódio-Hidrogênio / Mucosa Intestinal Idioma: En Ano de publicação: 2023 Tipo de documento: Article