The formation mechanism of Bacillus subtilis biofilm surface morphology under competitive environment.
Can J Microbiol
; 69(7): 251-261, 2023 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-36893426
ABSTRACT
Material properties and growth environments affect the surface morphology of biofilms. Taken the biofilm growing in competitive environments as the object, which is compared with the single biofilm, we find that the competitive environment has an impact on the biofilm thickness and wrinkle patterns. Through diffusion-limited growth theoretical model analysis, it shows that the competitive environment is caused by cells competing for nutrition, and the competitive environment reacts on biofilms, which affect the phenotypic differentiation, causing changes in the stiffness of the biofilm. Using the theoretical and finite element simulation, we compare these results of bi-layer and tri-layer film-substrate models with experimental observations, and find that tri-layer film-substrate model is in line with the reality, which means that the layer between the biofilm and substrate plays an import role for wrinkle formation. Based on the above analysis, we further study effects of biofilm stiffness and interlayer thickness on wrinkles under competitive environment.
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Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Biofilmes
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article