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Phenolic Profile, Antioxidant Activity, and Chemometric Classification of Carob Pulp and Products.
Ioannou, Georgia D; Savva, Ioanna K; Christou, Atalanti; Stavrou, Ioannis J; Kapnissi-Christodoulou, Constantina P.
Afiliação
  • Ioannou GD; Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
  • Savva IK; Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
  • Christou A; Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
  • Stavrou IJ; Department of Life Sciences, European University Cyprus, 2404 Nicosia, Cyprus.
  • Kapnissi-Christodoulou CP; Department of Chemistry, University of Cyprus, 1678 Nicosia, Cyprus.
Molecules ; 28(5)2023 Feb 28.
Article em En | MEDLINE | ID: mdl-36903513
In recent years, carob and its derived products have gained wide attention due to their health-promoting effects, which are mainly attributed to their phenolic compounds. Carob samples (carob pulps, powders, and syrups) were analyzed to investigate their phenolic profile using high-performance liquid chromatography (HPLC), with gallic acid and rutin being the most abundant compounds. Moreover, the antioxidant capacity and total phenolic content of the samples were estimated through DPPH (IC50 98.83-488.47 mg extract/mL), FRAP (48.58-144.32 µmol TE/g product), and Folin-Ciocalteu (7.20-23.18 mg GAE/g product) spectrophotometric assays. The effect of thermal treatment and geographical origin of carobs and carob-derived products on their phenolic composition was assessed. Both factors significantly affect the concentrations of secondary metabolites and, therefore, samples' antioxidant activity (p-value < 10-7). The obtained results (antioxidant activity and phenolic profile) were evaluated via chemometrics, through a preliminary principal component analysis (PCA) and orthogonal partial least square-discriminant analysis (OPLS-DA). The OPLS-DA model performed satisfactorily, differentiating all samples according to their matrix. Our results indicate that polyphenols and antioxidant capacity can be chemical markers for the classification of carob and its derived products.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fabaceae / Antioxidantes Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fabaceae / Antioxidantes Idioma: En Ano de publicação: 2023 Tipo de documento: Article