Challenges and opportunities for circRNA identification and delivery.
Crit Rev Biochem Mol Biol
; 58(1): 19-35, 2023 02.
Article
em En
| MEDLINE
| ID: mdl-36916323
ABSTRACT
Circular RNAs (circRNAs) are evolutionarily conserved noncoding RNAs with tissue-specific expression patterns, and exert unique cellular functions that have the potential to become biomarkers in therapeutic applications. Therefore, accurate and sensitive detection of circRNA with facile platforms is essential for better understanding of circRNA biological processes and circRNA-related disease diagnosis and prognosis; and precise regulation of circRNA through efficient delivery of circRNA or siRNA is critical for therapeutic purposes. Here, we reviewed the current development of circRNA identification methodologies, including overviewing the purification steps, summarizing the sequencing methods of circRNA, as well as comparing the advantages and disadvantages of traditional and new detection methods. Then, we discussed the delivery and manipulation strategies for circRNAs in both research and clinic treatment. Finally, the challenges and opportunities of analyzing circRNAs were addressed.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
RNA
/
RNA Circular
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article