Phenotypic characterization of Peripheral B cells in Mycobacterium tuberculosis infection and disease in Addis Ababa, Ethiopia.
Tuberculosis (Edinb)
; 140: 102329, 2023 05.
Article
em En
| MEDLINE
| ID: mdl-36921454
ABSTRACT
BACKGROUND:
Mortality and morbidity from tuberculosis (TB) remain one of the most important public health issues. Although cell-mediated immunity is the main immune response against Mycobacterium tuberculosis (MTB), the role of B-cells during MTB infection and disease is unclear.METHODS:
Peripheral blood mononuclear cells (PBMC) were isolated from treatment naïve Pulmonary TB patients (TB, nâ¯=â¯16), latent TB-infected participants (LTBI, nâ¯=â¯17), and healthy controls (HC, nâ¯=â¯19). PBMCs were stained with various fluorescently labeled antibodies to define B-cell subsets using multicolor flow cytometry.RESULTS:
Atypical memory B cells (CD19+CD27-CD21-) and circulating marginal zone B-cells (CD19+CD27+CD21+IgM+IgD+CD23-) were significantly higher in active TB when compared to LTBI and HC. CD5+ regulatory B cells (Breg, CD19+CD24hiCD38hiCD5+) and resting B-cells (CD19+CD27+CD21+) in Active TB patients were significantly lower compared to HC and LTBI. Overall, there were no differences in B cell percentages (CD19+), naïve B cells (CD19+CD27-CD21+), Breg (CD19+CD24hiCD38hi), and activated memory B cells (CD19+CD27+CD21-) among the three study groups.CONCLUSIONS:
These results indicated that multiple subsets of B cells were associated with TB infection and disease. It will be useful to examine these cell populations for their potential use as biomarkers for TB disease and LTBI.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Tuberculose
/
Tuberculose Latente
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Linfócitos B Reguladores
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Mycobacterium tuberculosis
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article