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Sperm Cryopreservation of the African Turquoise Killifish Nothobranchius furzeri.
Dolfi, Luca; Suen, Tsz Kin; Medelbekova, Danel; Ripa, Roberto; Symmons, Orsolya; Antebi, Adam.
Afiliação
  • Dolfi L; Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany.
  • Suen TK; Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany.
  • Medelbekova D; Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany.
  • Ripa R; Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany.
  • Symmons O; Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany.
  • Antebi A; Max Planck Institute for Biology of Ageing, 50931 Cologne, Germany aantebi@age.mpg.de.
Cold Spring Harb Protoc ; 2023(11): pdb.prot107885, 2023 Nov 01.
Article em En | MEDLINE | ID: mdl-36921999
ABSTRACT
Sperm cryopreservation is an essential method for the genetic preservation and long-term storage of wild-type and transgenic animal stocks. In addition, it allows for the synchronization of gamete availability and the transport and sharing of lines between different laboratories. Here, we describe a protocol developed in our laboratory for the extraction and cryopreservation of killifish (Nothobranchius furzeri) sperm.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fundulidae Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fundulidae Idioma: En Ano de publicação: 2023 Tipo de documento: Article