Tautomerisation Mechanisms in the Adenine-Thymine Nucleobase Pair during DNA Strand Separation.
J Phys Chem B
; 127(19): 4220-4228, 2023 05 18.
Article
em En
| MEDLINE
| ID: mdl-36939840
ABSTRACT
The adenine-thymine tautomer (A*-T*) has previously been discounted as a spontaneous mutagenesis mechanism due to the energetic instability of the tautomeric configuration. We study the stability of A*-T* while the nucleobases undergo DNA strand separation. Our calculations indicate an increase in the stability of A*-T* as the DNA strands unzip and the hydrogen bonds between the bases stretch. Molecular Dynamics simulations reveal the time scales and dynamics of DNA strand separation and the statistical ensemble of opening angles present in a biological environment. Our results demonstrate that the unwinding of DNA, an inherently out-of-equilibrium process facilitated by helicase, will change the energy landscape of the adenine-thymine tautomerization reaction. We propose that DNA strand separation allows the stable tautomerization of adenine-thymine, providing a feasible pathway for genetic point mutations via proton transfer between the A-T bases.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Timina
/
Adenina
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article