Pan-yellow fever virus detection and lineage assignment by real-time RT-PCR and amplicon sequencing.
J Virol Methods
; 316: 114717, 2023 06.
Article
em En
| MEDLINE
| ID: mdl-36972832
ABSTRACT
Yellow fever disease is a viral zoonosis that may result in a severe hemorrhagic disease. A safe and effective vaccine used in mass immunization campaigns has allowed control and mitigation against explosive outbreaks in endemic areas. Since the 1960's, re-emergent of the yellow fever virus has been observed. The timely implementation of control measures, to avoid or contain an ongoing outbreak requires rapid specific viral detection methods. Here a novel molecular assay, expected to detect all known yellow fever virus strains, is described. The method has demonstrated high sensitivity and specificity in real-time RT-PCR as well as in an endpoint RT-PCR set-up. Sequence alignment and phylogenetic analysis reveal that the amplicon resulting from the novel method covers a genomic region whose mutational profile is completely associated to the yellow fever viral lineages. Therefore, sequencing analysis of this amplicon allows for assignment of the viral lineage.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Febre Amarela
/
Vacina contra Febre Amarela
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article