BIND&MODIFY: a long-range method for single-molecule mapping of chromatin modifications in eukaryotes.
Genome Biol
; 24(1): 61, 2023 03 29.
Article
em En
| MEDLINE
| ID: mdl-36991510
ABSTRACT
Epigenetic modifications of histones are associated with development and pathogenesis of disease. Existing approaches cannot provide insights into long-range interactions and represent the average chromatin state. Here we describe BIND&MODIFY, a method using long-read sequencing for profiling histone modifications and transcription factors on individual DNA fibers. We use recombinant fused protein A-M.EcoGII to tether methyltransferase M.EcoGII to protein binding sites to label neighboring regions by methylation. Aggregated BIND&MODIFY signal matches bulk ChIP-seq and CUT&TAG. BIND&MODIFY can simultaneously measure histone modification status, transcription factor binding, and CpG 5mC methylation at single-molecule resolution and also quantifies correlation between local and distal elements.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Eucariotos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article