Tsuneonella litorea sp. nov., a novel carotenoid-producing bacterium isolated from coastal sediment.
Int J Syst Evol Microbiol
; 73(3)2023 Mar.
Article
em En
| MEDLINE
| ID: mdl-37009855
ABSTRACT
A Gram-stain-negative, aerobic, non-motile and pleomorphic bacterium designated as YG55T was isolated from a coastal sediment sample. Growth was found to occur at 10-37 °C (optimum, 28 °C), at pH 6-9 (optimum, pH 8) and in 0-6â% NaCl (optimum, 1â%). The results of 16S rRNA gene-based analysis showed that strain YG55T was related to the members of the genus Tsuneonella and shared the highest identity of 99.4â% with Tsuneonella dongtanensis GDMCC 1.2307T, followed by Tsuneonella troitsensis JCM 17037T (98.4â%). The phylogenomic results indicated that strain YG55T formed an independent branch distinct from the reference type strains. The 22.7 and 21.8â% digital DNA-DNA hybridization (dDDH) values and 83.0 and 81.8â% average nucleotide identity (ANI) values between strain YG55T and the two relatives were below the species definition thresholds of 70â% (dDDH) and 95-96â% (ANI), indicating that the strain represents a novel genospecies. The results of chemotaxonomic characterization indicated that the major cellular fatty acids of strain YG55T were summed feature 8 (C18ââ1 ω6c and/or C18ââ1 ω7c), C14ââ0 2OH and C16ââ0; the main polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; the respiratory quinone was ubiquinone-10. The genomic size and DNA G+C contents were 3.03 Mbp and 66.98â%. The strain contained carotenoid biosynthesis genes and could produce carotenoids. Based on its genotypic and phenotypic characteristics, strain YG55T is concluded to represent a novel species of the genus Tsuneonella, for which the name Tsuneonella litorea sp. nov. is proposed. The type strain is YG55T (=GDMCC 1.2590 T=KCTC 82812T).
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Fosfolipídeos
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Ácidos Graxos
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En
Ano de publicação:
2023
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Article