The T2 structure of polycrystalline cubic human insulin.
Acta Crystallogr D Struct Biol
; 79(Pt 5): 374-386, 2023 May 01.
Article
em En
| MEDLINE
| ID: mdl-37039669
ABSTRACT
The polymorphism of human insulin upon pH variation was characterized via X-ray powder diffraction, employing a crystallization protocol previously established for co-crystallization with phenolic derivatives. Two distinct rhombohedral (R3) polymorphs and one cubic (I213) polymorph were identified with increasing pH, corresponding to the T6, T3R3f and T2 conformations of insulin, respectively. The structure of the cubic T2 polymorph was determined via multi-profile stereochemically restrained Rietveld refinement at 2.7â
Å resolution. This constitutes the first cubic insulin structure to be determined from crystals grown in the presence of zinc ions, although no zinc binding was observed. The differences of the polycrystalline variant from other cubic insulin structures, as well as the nature of the pH-driven phase transitions, are discussed in detail.
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Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Insulina Regular Humana
/
Insulina
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article