Contractile effect of trimethylamine and trimethylamine-n-oxide on isolated human umbilical arteries.
J Obstet Gynaecol Res
; 49(7): 1736-1742, 2023 Jul.
Article
em En
| MEDLINE
| ID: mdl-37045561
ABSTRACT
BACKGROUND:
The aim of this study is to investigate the effect of trimethylamine (TMA) and trimethylamine-n-oxide (TMAO) on the contractility of human umbilical artery and the possible mechanisms involved.METHODS:
Vasoactive responses to TMA and TMAO on human umbilical artery rings were measured in isolated organ baths. Cumulative dose-response curves for TMA and TMAO were obtained before and after incubation with atropine, yohimbine, prazosin, indomethacin, verapamil, and Ca+2 -free Krebs-Henselite solution.RESULTS:
Administration of cumulative TMA and TMAO resulted in dose-dependent contraction at concentrations ranging from 10 to 100 mM on human umbilical artery rings. TMA-induced contractions were more potent than TMAO-induced contractions (TMA -logEC50 = 1.00 ± 0.02, TMAO -logEC50 = 0.57 ± 0.02). Contraction responses to TMA were significantly lower in the presence of verapamil and in the absence of external Ca+2 (p < 0.001, p < 0.05, respectively).CONCLUSION:
Our results showed that TMA and TMAO caused vasoconstriction in isolated human umbilical artery rings. Our findings also indicated that TMA but not TMAO-induced vasoconstriction was partially dependent on extracellular Ca2+ and calcium influx through L-type Ca2+ channels. Our results suggest that TMA and TMAO may have the potential to contribute to cardiovascular diseases through their direct effect on vascular contractility in human arteries.Palavras-chave
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Base de dados:
MEDLINE
Assunto principal:
Artérias Umbilicais
/
Metilaminas
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article