Assessment of Bioenergetics in BAT Mitochondria.
Methods Mol Biol
; 2662: 67-75, 2023.
Article
em En
| MEDLINE
| ID: mdl-37076671
ABSTRACT
High-resolution respirometry is commonly used to quantify mitochondrial respiratory rates. In the respirometry chamber, a change in oxygen concentration is measured by a polarographic electrode to derive the rate of oxygen consumption (JO2). Here, we describe our adapted protocol to bioenergetically phenotype mitochondria from mouse brown adipose tissue (BAT). Given the presence of uncoupling protein 1 (UCP1), mitochondria from BAT provide unique challenges and opportunities in applying high-resolution respirometry to understand energy transduction through oxidative phosphorylation (OXPHOS).
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Base de dados:
MEDLINE
Assunto principal:
Metabolismo Energético
/
Mitocôndrias
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article