Comprehensive RNA-seq profiling to evaluate the rabbit mammary gland transcriptome after mastitis.

ABSTRACT

Mastitis is a relatively common disease in rabbit does. The aim of this study was to investigate a relationship between the severity of clinical signs and pathological observations and to analyze differentially expressed genes (DEGs) in the mammary gland with mastitis versus healthy mammary gland. The result showed that rectal temperatures of the rabbits with both mild mastitis and severe mastitis were higher than that of control. Cell counting results showed that the somatic cell count (SCC) only in milk of the rabbit with severe mastitis was significantly higher than that in the control group. However, the number of heterophils in the histological sections of mammary glands with mild mastitis was significantly higher than that of control. A total of 1,096 DEGs between the control and mastitis mammary glands was identified by RNA-sequencing (RNA-seq). Gene ontology (GO) showed that most of up-regulated genes were enriched in terms such as response to stimulus, signal transduction, and cell communication. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these genes were mostly enriched in the pathways such as Rap1 signaling pathway, proteoglycans in cancer, and PI3K-Akt signaling pathway. However, the downregulated genes were mainly enriched in metabolic processes and significantly involved in metabolic pathways. The data provides useful information to further dissect the molecular genetic mechanisms underlying rabbit mastitis, which is a prerequisite for designing effective intervention strategies.