Combined FRET-FLIM and NAD(P)H FLIM to Analyze B Cell Receptor Signaling Induced Metabolic Activity of Germinal Center B Cells In Vivo.

Ulbricht, Carolin; Leben, Ruth; Cao, Yu; Niesner, Raluca A; Hauser, Anja E

Ulbricht, Carolin; Leben, Ruth; Cao, Yu; Niesner, Raluca A; Hauser, Anja E.

Afiliação

Ulbricht C; Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Rheumatology and Clinical Immunology, Berlin, Germany.
Leben R; Immune Dynamics, Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute, Berlin, Germany.
Cao Y; Biophysical Analysis, Deutsches Rheuma-Forschungszentrum (DRFZ), a Leibniz Institute, Berlin, Germany.
Niesner RA; Dynamic and functional in vivo imaging, Freie Universität Berlin, Veterinary Medicine, Berlin, Germany.
Hauser AE; Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Rheumatology and Clinical Immunology, Berlin, Germany.

Methods Mol Biol ; 2654: 91-111, 2023.

Article em En | MEDLINE | ID: mdl-37106177

Assuntos

Cálcio; NAD; NAD/metabolismo; Cálcio/metabolismo; Transferência Ressonante de Energia de Fluorescência; Centro Germinativo; Receptores de Antígenos de Linfócitos B/metabolismo

Palavras-chave

B cell receptor signaling; B cells; Fluorescence lifetime microscopy; Germinal center; Intravital microscopy; Metabolic reprogramming; NADPH oxidase

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cálcio / NAD Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Cálcio / NAD Idioma: En Ano de publicação: 2023 Tipo de documento: Article