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Protocol for electrotaxis of large epithelial cell sheets.
Zhang, Yan; Lee, Rachel M; Zhu, Zijie; Sun, Yaohui; Zhu, Kan; Xu, Zhengping; Lin, Francis; Pan, Tingrui; Losert, Wolfgang; Zhao, Min.
Afiliação
  • Zhang Y; Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; School of Public Health, Hangzhou Normal University, Hangzhou 311121, China; Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China; Micro-Nano I
  • Lee RM; Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA.
  • Zhu Z; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA.
  • Sun Y; Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA.
  • Zhu K; Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA.
  • Xu Z; Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
  • Lin F; Department of Physics and Astronomy, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
  • Pan T; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA; Institute of Biomedical and Health Engineering, Shenzhen Institute of Advanced Technology, Shenzhen 518055, China; Shenzhen Engineering Laboratory of Single-mol
  • Losert W; Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA; Department of Physics, University of Maryland, College Park, MD 20742, USA.
  • Zhao M; Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; Department of Dermatology, University of California, California, Davis, CA 95616, USA. Electronic address: minzhao@ucdavis.edu.
STAR Protoc ; 4(2): 102288, 2023 May 06.
Article em En | MEDLINE | ID: mdl-37149857
ABSTRACT
Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article