H2O2 selectively damages the binuclear iron-sulfur cluster N1b of respiratory complex I.
Sci Rep
; 13(1): 7652, 2023 05 11.
Article
em En
| MEDLINE
| ID: mdl-37169846
ABSTRACT
NADHubiquinone oxidoreductase, respiratory complex I, plays a major role in cellular energy metabolism by coupling electron transfer with proton translocation. Electron transfer is catalyzed by a flavin mononucleotide and a series of iron-sulfur (Fe/S) clusters. As a by-product of the reaction, the reduced flavin generates reactive oxygen species (ROS). It was suggested that the ROS generated by the respiratory chain in general could damage the Fe/S clusters of the complex. Here, we show that the binuclear Fe/S cluster N1b is specifically damaged by H2O2, however, only at high concentrations. But under the same conditions, the activity of the complex is hardly affected, since N1b can be easily bypassed during electron transfer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Complexo I de Transporte de Elétrons
/
Proteínas Ferro-Enxofre
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article