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NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens.
Bogdanova, Yulia A; Zaitseva, Elvira R; Smirnov, Alexander Yu; Baleeva, Nadezhda S; Gavrikov, Alexey S; Myasnyanko, Ivan N; Goncharuk, Sergey A; Kot, Erik F; Mineev, Konstantin S; Mishin, Alexander S; Baranov, Mikhail S.
Afiliação
  • Bogdanova YA; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Zaitseva ER; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Smirnov AY; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Baleeva NS; Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia.
  • Gavrikov AS; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Myasnyanko IN; Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia.
  • Goncharuk SA; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Kot EF; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Mineev KS; Laboratory of Medicinal Substances Chemistry, Institute of Translational Medicine, Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia.
  • Mishin AS; Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
  • Baranov MS; Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.
Int J Mol Sci ; 24(9)2023 Apr 27.
Article em En | MEDLINE | ID: mdl-37175667
ABSTRACT
In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Corantes Fluorescentes Idioma: En Ano de publicação: 2023 Tipo de documento: Article
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Corantes Fluorescentes Idioma: En Ano de publicação: 2023 Tipo de documento: Article