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A goat eye, wet lab model for training in Descemet membrane endothelial keratoplasty.
Gupta, Nidhi; Bhogal, Maninder; Vaddavalli, Pravin K; Boldini, Alessandro; Semeraro, Francesco; Varshney, Akhil; Romano, Vito.
Afiliação
  • Gupta N; Department of Cornea and Refractive Services, and Stem Cell Lab, Dr. Shroff's Charity Eye Hospital, Delhi, India.
  • Bhogal M; Cornea Unit, Guy's and St. Thomas' Hospital, London, UK.
  • Vaddavalli PK; The Cornea Institute, L V Prasad Eye Institute, Hyderabad, Telangana, India.
  • Boldini A; Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, Ophthalmology Clinic, University of Brescia, Italy.
  • Semeraro F; Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, Ophthalmology Clinic, University of Brescia, Italy.
  • Varshney A; Department of Cornea and Refractive Services, and Stem Cell Lab, Dr. Shroff's Charity Eye Hospital, Delhi, India.
  • Romano V; Department of Medical and Surgical Specialties, Radiological Sciences, and Public Health, Ophthalmology Clinic, University of Brescia, Italy; Department of Eye and Vision Science, Institute of Life Course and Medical Sciences, University of Liverpool, Liverpool, UK.
Indian J Ophthalmol ; 71(5): 2230-2233, 2023 05.
Article em En | MEDLINE | ID: mdl-37202956
Here we describe a new, non-human, ex-vivo model (goat eye model) for training surgeons in DMEK surgeons. In a wet lab setting, goat eyes were used to obtain a pseudo-DMEK graft of 8 mm from the goat lens capsule that was injected into another goat eye with the same maneuvers described for human DMEK. The DMEK pseudo-graft can be easily prepared, stained, loaded, injected, and unfolded into the goat eye model reproducing the similar maneuvers used for DMEK in a human eye, except for the descemetorhexis, which cannot be performed. The pseudo-DMEK graft behaves similar to human DMEK graft and useful for surgeons to experience and understand steps of DMEK early in learning curve. The concept of a non-human ex-vivo eye model is simple and reproducible and obviates the need for human tissue and the issues of poor visibility in stored corneal tissue.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Distrofia Endotelial de Fuchs / Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Distrofia Endotelial de Fuchs / Ceratoplastia Endotelial com Remoção da Lâmina Limitante Posterior Idioma: En Ano de publicação: 2023 Tipo de documento: Article