High-throughput, highly sensitive and rapid SERS detection of Escherichia coli O157:H7 using aptamer-modified Au@macroporous silica magnetic photonic microsphere array.
Food Chem
; 424: 136433, 2023 Oct 30.
Article
em En
| MEDLINE
| ID: mdl-37244192
ABSTRACT
The aim of this research was to develop a simple, rapid, sensitive, high-throughput detection method for foodborne Escherichia coli (E. coli) O157H7 based on the aptamer-modified gold nanoparticles@macroporous magnetic silica photonic microsphere (Au@MMSPM). Such Au@MMSPM array system for E. coli O157H7 not only integrated sample pretreatment with rapid detection, but also showed highly enhanced effect to develop a highly sensitive SERS assay. The established SERS assay platform gave a wide linear detection range (10-106 CFU/mL) and low limit of detection (2.20 CFU/mL) for E. coli O157H7. The whole analysis time including sample pretreatment and detection was 110 min. This SERS-based assay platform provided a new high-throughput, highly sensitive and fast detection technology for monitoring E. coli O157H7 in real samples from the fields of food industry, medicine and environment.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
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Escherichia coli O157
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Nanopartículas Metálicas
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article