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Diadenosine pentaphosphate regulates dendrite growth and number in cultured hippocampal neurons.
Diez-Zaera, M; Ruiz-Calvo, A; Diaz-Hernandez, J I; Sebastián-Serrano, A; Aivar, P; Alvarez-Castelao, B; Pintor, J; Diaz-Hernandez, M; Miras-Portugal, M T.
Afiliação
  • Diez-Zaera M; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain.
  • Ruiz-Calvo A; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain.
  • Diaz-Hernandez JI; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain.
  • Sebastián-Serrano A; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain.
  • Aivar P; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain.
  • Alvarez-Castelao B; Departamento Ciencia de La Salud, Facultad Ciencias Biomédicas y de La Salud, Universidad Europea de Madrid, 28670, Madrid, Spain.
  • Pintor J; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain.
  • Diaz-Hernandez M; Departamento de Bioquímica y Biología Molecular, Facultad de Óptica y Optometría, Universidad Complutense de Madrid, 28037, Madrid, Spain.
  • Miras-Portugal MT; Departamento de Bioquímica y Biología Molecular, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro S/N, 28040, Madrid, Spain. migueldiaz@ucm.es.
Purinergic Signal ; 20(2): 115-125, 2024 Apr.
Article em En | MEDLINE | ID: mdl-37246192
During the establishment of neuronal circuits, axons and dendrites grow and branch to establish specific synaptic connections. This complex process is highly regulated by positive and negative extracellular cues guiding the axons and dendrites. Our group was pioneer in describing that one of these signals are the extracellular purines. We found that extracellular ATP, through its selective ionotropic P2X7 receptor (P2X7R), negatively regulates axonal growth and branching. Here, we evaluate if other purinergic compounds, such as the diadenosine pentaphosphate (Ap5A), may module the dynamics of dendritic or axonal growth and branching in cultured hippocampal neurons. Our results show that Ap5A negatively modulates the dendrite's growth and number by inducing transient intracellular calcium increases in the dendrites' growth cone. Interestingly, phenol red, commonly used as a pH indicator in culture media, also blocks the P2X1 receptors, avoided the negative modulation of Ap5A on dendrites. Subsequent pharmacological studies using a battery of selective P2X1R antagonists confirmed the involvement of this subunit. In agreement with pharmacological studies, P2X1R overexpression caused a similar reduction in dendritic length and number as that induced by Ap5A. This effect was reverted when neurons were co-transfected with the vector expressing the interference RNA for P2X1R. Despite small hairpin RNAs reverting the reduction in the number of dendrites caused by Ap5A, it did not avoid the dendritic length decrease induced by the polyphosphate, suggesting, therefore, the involvement of a heteromeric P2X receptor. Our results are indicating that Ap5A exerts a negative influence on dendritic growth.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfatos de Dinucleosídeos / Trifosfato de Adenosina / Receptores Purinérgicos P2 Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fosfatos de Dinucleosídeos / Trifosfato de Adenosina / Receptores Purinérgicos P2 Idioma: En Ano de publicação: 2024 Tipo de documento: Article