Effect of Solid-State Fermented Brown Rice Extracts on 3T3-L1 Adipocyte Differentiation.
J Microbiol Biotechnol
; 33(7): 926-933, 2023 Jul 28.
Article
em En
| MEDLINE
| ID: mdl-37254304
ABSTRACT
Aspergillus oryzae KCCM 11372 was used to enhance the production of ß-glucan using humidity control strategies. Under conditions of 60% humidity, solid-state fermentation (SSF) increased the yields of enzymes (amylase and protease), fungal biomass (ergosterol), and ß-glucan. The maximum concentrations obtained were 14800.58 U/g at 72 h, 1068.14 U/g at 120 h, 1.42 mg/g at 72 h, and 12.0% (w/w) at 72 h, respectively. Moreover, the ß-glucan containing fermented brown rice (ß-glucan-FBR) extracts at concentrations of 25-300 µg/ml was considered noncytotoxic to 3T3-L1 preadipocytes. We then studied the inhibitory effects of the extracts on fat droplet formation in 3T3-L1 cells. As a result, 300 µg/ml of ß-glucan-FBR extracts showed a high inhibition of 38.88% in lipid accumulation. Further, these extracts inhibited adipogenesis in the 3T3-L1 adipocytes by decreasing the expression of C/EBPα, PPARγ, aP2, and GLUT4 genes.
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1
Base de dados:
MEDLINE
Assunto principal:
Oryza
/
Beta-Glucanas
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article