Construction of a Temperature-Sensitive Shuttle Vector between Lactic Acid Bacteria and Escherichia coli and Its Application to a Tn10-Based Random Mutagenesis Tool in Lactic Acid Bacteria.

Noda, Masafumi; Kumagai, Takanori; Yamaoka, Marina; Danshiitsoodol, Narandalai; Sugiyama, Masanori

Noda, Masafumi; Kumagai, Takanori; Yamaoka, Marina; Danshiitsoodol, Narandalai; Sugiyama, Masanori.

Afiliação

Noda M; Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Kumagai T; Department of Microbiology, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Yamaoka M; Department of Microbiology, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Danshiitsoodol N; Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Sugiyama M; Department of Probiotic Science for Preventive Medicine, Graduate School of Biomedical and Health Sciences, Hiroshima University.

Biol Pharm Bull ; 46(6): 840-847, 2023.

Article em En | MEDLINE | ID: mdl-37258150

Assuntos

Escherichia coli; Lactobacillales; Escherichia coli/genética; Escherichia coli/metabolismo; Temperatura; Replicação do DNA; Vetores Genéticos/genética; Plasmídeos/genética; Proteínas/metabolismo; Bactérias/genética; Mutagênese; Ácido Láctico/metabolismo

Palavras-chave

lactic acid bacteria; plasmid; random mutagenesis; temperature-sensitive; transposon

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Lactobacillales Idioma: En Ano de publicação: 2023 Tipo de documento: Article

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PubMed Links

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Escherichia coli / Lactobacillales Idioma: En Ano de publicação: 2023 Tipo de documento: Article