Whole Cell Patch Clamp Electrophysiology in Human Neuronal Cells.
Methods Mol Biol
; 2683: 259-273, 2023.
Article
em En
| MEDLINE
| ID: mdl-37300782
ABSTRACT
Whole cell patch clamp recording techniques are commonly used to assay membrane excitability, ion channel function, and synaptic activity in neurons. However, assaying these functional properties of human neurons remains difficult because of the difficulty in obtaining human neuronal cells. Recent advents in stem cell biology, especially the development of the induced pluripotent stem cells, made it possible to generate human neuronal cells in both 2-dimensional (2D) monolayer cultures and 3D brain-organoid cultures. Here, we describe the whole cell patch clamp methods of recording neuronal physiology from human neuronal cells.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes Induzidas
/
Neurônios
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article