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Not always an innocent bystander: the impact of stabilised phosphopantetheine moieties when studying nonribosomal peptide biosynthesis.
Ho, Y T Candace; Kaczmarski, Joe A; Tailhades, Julien; Izoré, Thierry; Steer, David L; Schittenhelm, Ralf B; Tosin, Manuela; Jackson, Colin J; Cryle, Max J.
Afiliação
  • Ho YTC; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia. max.cryle@monash.edu.
  • Kaczmarski JA; EMBL Australia, Monash University, Clayton, VIC 3800, Australia.
  • Tailhades J; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Australia.
  • Izoré T; Department of Chemistry, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK.
  • Steer DL; ARC Centre of Excellence in Synthetic Biology, Australian National University, Canberra, Australia.
  • Schittenhelm RB; Research School of Biology, Australian National University, Acton, Australian Capital Territory, Australia.
  • Tosin M; Department of Biochemistry and Molecular Biology, The Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC 3800, Australia. max.cryle@monash.edu.
  • Jackson CJ; EMBL Australia, Monash University, Clayton, VIC 3800, Australia.
  • Cryle MJ; ARC Centre of Excellence for Innovations in Peptide and Protein Science, Australia.
Chem Commun (Camb) ; 59(53): 8234-8237, 2023 Jun 29.
Article em En | MEDLINE | ID: mdl-37310188
ABSTRACT
Nonribosomal peptide synthetases produce many important peptide natural products and are centred around carrier proteins (CPs) that deliver intermediates to various catalytic domains. We show that the replacement of CP substrate thioesters by stabilised ester analogues leads to active condensation domain complexes, whereas amide stabilisation generates non-functional complexes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Sintases / Biossíntese de Peptídeos Independentes de Ácido Nucleico Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeo Sintases / Biossíntese de Peptídeos Independentes de Ácido Nucleico Idioma: En Ano de publicação: 2023 Tipo de documento: Article