Protocol for purification of cells in their native state using reversible aptamer-antidote pairs.
STAR Protoc
; 4(3): 102348, 2023 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-37314924
ABSTRACT
Cell isolation from complex mixtures is a key step in many clinical and research applications, but standard isolation methods may affect the cell's biology and are difficult to reverse. Here, we present a method to isolate and restore cells to their native state using an aptamer that binds epidermal growth factor receptor (EGFR+)cells and a complementary antisense oligonucleotide to reverse binding. For complete details on the use and execution of this protocol, please refer to Gray et al.1.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Antídotos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article