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Occurrence of shigellosis in pediatric diarrheal patients in Chattogram, Bangladesh: A molecular based approach.
Zakir Hossain, A K M; Zahid Hasan, Md; Mina, Sohana Akter; Sultana, Nahid; Chowdhury, A M Masudul Azad.
Afiliação
  • Zakir Hossain AKM; Department of Genetic Engineering & Biotechnology, Laboratory of Microbial & Cancer Genomics, University of Chittagong, Bangladesh.
  • Zahid Hasan M; Department of Genetic Engineering & Biotechnology, Laboratory of Microbial & Cancer Genomics, University of Chittagong, Bangladesh.
  • Mina SA; Department of Genetic Engineering & Biotechnology, Laboratory of Microbial & Cancer Genomics, University of Chittagong, Bangladesh.
  • Sultana N; Department of Microbiology, Chattogram Maa-O-Shishu Hospital Medical College, Chattogram, Bangladesh.
  • Chowdhury AMMA; Department of Genetic Engineering & Biotechnology, Laboratory of Microbial & Cancer Genomics, University of Chittagong, Bangladesh.
PLoS One ; 18(6): e0275353, 2023.
Article em En | MEDLINE | ID: mdl-37319254
Shigellaa Gram-negative, non-motile bacillus, is the primary causative agent of the infectious disease shigellosis, which kills 1.1 million people worldwideevery year. The children under the age of five are primarily the victims of this disease. This study has been conducted to assess the prevalence of shigellosis through selective plating, biochemical test and conventional PCR assays, where the samples were collected from suspected diarrheoal patients. Invasive plasmid antigen H (ipaH) and O-antigenic rfc gene were used to identify Shigella spp. and S. flexneri respectively. For validation of these identification, PCR product of ipaH gene of a sample (Shigella flexneri MZS 191) has been sequenced and submitted to NCBI database (GenBank accession no- MW774908.1). Further this strain has been used as positive control. Out of 204, around 14.2% (n = 29)(P> 0.01) pediatric diarrheoal cases were screened as shigellosis. Another interesting finding was that most of shigellosis affected children were 7 months to 1 year (P> 0.01).The significance of this study lies in the analyses of the occurrenceand the molecular identification of Shigellaspp. and S. flexneri that can be utilized in improving the accurate identification and the treatment of the most severe and alarming shigellosis.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Shigella / Disenteria Bacilar Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Shigella / Disenteria Bacilar Idioma: En Ano de publicação: 2023 Tipo de documento: Article