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Protocol for optimizing production and quality control of infective EcoHIV virions.
Alfar, Hammodah R; Pariser, Daphne N; Chanzu, Harry; Joshi, Smita; Coenen, Daniëlle M; Lykins, Joshua; Prakhya, Kanakanagavalli Shravani; Potash, Mary Jane; Chao, Wei; Kelschenbach, Jennifer; Volsky, David J; Metcalf-Pate, Kelly; Whiteheart, Sidney W.
Afiliação
  • Alfar HR; University of Kentucky, College of Medicine, Lexington, KY 40506, USA.
  • Pariser DN; Massachusetts Institute of Technology, Division of Comparative Medicine, 77 Massachusetts Avenue, Cambridge, MA 02139, USA; Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
  • Chanzu H; University of Kentucky, College of Medicine, Lexington, KY 40506, USA.
  • Joshi S; University of Kentucky, College of Medicine, Lexington, KY 40506, USA.
  • Coenen DM; University of Kentucky, College of Medicine, Lexington, KY 40506, USA.
  • Lykins J; University of Kentucky, College of Medicine, Lexington, KY 40506, USA.
  • Prakhya KS; University of Kentucky, College of Medicine, Lexington, KY 40506, USA.
  • Potash MJ; Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Pl, New York, NY 10029, USA.
  • Chao W; Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Pl, New York, NY 10029, USA.
  • Kelschenbach J; Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Pl, New York, NY 10029, USA.
  • Volsky DJ; Icahn School of Medicine at Mount Sinai, 1 Gustave L. Levy Pl, New York, NY 10029, USA.
  • Metcalf-Pate K; Massachusetts Institute of Technology, Division of Comparative Medicine, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.
  • Whiteheart SW; University of Kentucky, College of Medicine, Lexington, KY 40506, USA. Electronic address: whitehe@uky.edu.
STAR Protoc ; 4(3): 102368, 2023 Sep 15.
Article em En | MEDLINE | ID: mdl-37342907
ABSTRACT
EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por HIV / HIV-1 Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Infecções por HIV / HIV-1 Idioma: En Ano de publicação: 2023 Tipo de documento: Article