Fabrication of cell-laden microbeads and microcapsules composed of bacterial polyglucuronic acid.
Int J Biol Macromol
; 244: 125481, 2023 Jul 31.
Article
em En
| MEDLINE
| ID: mdl-37343612
ABSTRACT
In the past decades, the microencapsulation of mammalian cells into microparticles has been extensively studied for various in vitro and in vivo applications. The aim of this study was to demonstrate the viability of bacterial polyglucuronic acid (PGU), an exopolysaccharide derived from bacteria and composed of glucuronic acid units, as an effective material for cell microencapsulation. Using the method of dropping an aqueous solution of PGU-containing cells into a Ca2+-loaded solution, we produced spherical PGU microbeads with >93 % viability of the encapsulated human hepatoma HepG2 cells. Hollow-core microcapsules were formed via polyelectrolyte complex layer formation of PGU and poly-l-lysine, after which Ca2+, a cross-linker of PGU, was chelated, and this was accomplished by sequential immersion of microbeads in aqueous solutions of poly-l-lysine and sodium citrate. The encapsulated HepG2 cells proliferated and formed cell aggregates within the microparticles over a 14-day culture, with significantly larger aggregates forming within the microcapsules. Our results provide evidence for the viability of PGU for cell microencapsulation for the first time, thereby contributing to advancements in tissue engineering.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Polilisina
/
Engenharia Tecidual
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article