Using Ion Substitution and Fluid Indicators to Monitor Macropinosome Dynamics in Live Cells.
Methods Mol Biol
; 2692: 375-384, 2023.
Article
em En
| MEDLINE
| ID: mdl-37365480
ABSTRACT
All forms of endocytosis involve the incidental uptake of fluid (pinocytosis). Macropinocytosis is a specialized type of endocytosis that results in the bulk ingestion of extracellular fluid via large (>0.2 µm) vacuoles called macropinosomes. The process is a means of immune surveillance, a point of entry for intracellular pathogens, and a source of nutrients for proliferating cancer cells. Macropinocytosis has also recently emerged as a tractable system that can be experimentally exploited to understand fluid handling in the endocytic pathway. In this chapter, we describe how stimulating macropinocytosis in the presence of extracellular fluids of a defined ionic composition can be combined with high-resolution microscopy to understand the role of ion transport in controlling membrane traffic.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Pinocitose
/
Endocitose
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article