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Mechanistic insights into transposon cleavage and integration by TnsB of ShCAST system.
Zeng, Ting; Yin, Jie; Liu, Ziwen; Li, Zhaoxing; Zhang, Yu; Lv, Yang; Lu, Mei-Ling; Luo, Min; Chen, Meirong; Xiao, Yibei.
Afiliação
  • Zeng T; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • Yin J; Department of Biochemistry, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
  • Liu Z; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • Li Z; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • Zhang Y; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • Lv Y; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China.
  • Lu ML; Department of Biochemistry, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China.
  • Luo M; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.
  • Chen M; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China. Electronic address: chenmr@cpu.edu.cn.
  • Xiao Y; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China; Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 210009, China; Chongqing Innovation Institute of China Pharmaceutical University, Chongqing 401135, China. Elect
Cell Rep ; 42(7): 112698, 2023 07 25.
Article em En | MEDLINE | ID: mdl-37379212
ABSTRACT
The type V-K CRISPR-associated transposons (CASTs) allow RNA-guided DNA integration and have great potential as a programmable site-specific gene insertion tool. Although all core components have been independently characterized structurally, the mechanism of how the transposase TnsB associates with AAA+ ATPase TnsC and catalyzes donor DNA cleavage and integration remains ambiguous. In this study, we demonstrate that TniQ-dCas9 fusion can direct site-specific transposition by TnsB/TnsC in ShCAST. TnsB is a 3'-5' exonuclease that specifically cleaves donor DNA at the end of the terminal repeats and integrates the left end prior to the right end. The nucleotide preference and the cleavage site of TnsB are markedly different from those of the well-documented MuA. We also find that TnsB/TnsC association is enhanced in a half-integration state. Overall, our results provide valuable insights into the mechanism and application expansion of CRISPR-mediated site-specific transposition by TnsB/TnsC.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Proteínas de Escherichia coli Idioma: En Ano de publicação: 2023 Tipo de documento: Article