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Spatial mapping of cellular senescence: emerging challenges and opportunities.
Gurkar, Aditi U; Gerencser, Akos A; Mora, Ana L; Nelson, Andrew C; Zhang, Anru R; Lagnado, Anthony B; Enninful, Archibald; Benz, Christopher; Furman, David; Beaulieu, Delphine; Jurk, Diana; Thompson, Elizabeth L; Wu, Fei; Rodriguez, Fernanda; Barthel, Grant; Chen, Hao; Phatnani, Hemali; Heckenbach, Indra; Chuang, Jeffrey H; Horrell, Jeremy; Petrescu, Joana; Alder, Jonathan K; Lee, Jun Hee; Niedernhofer, Laura J; Kumar, Manoj; Königshoff, Melanie; Bueno, Marta; Sokka, Miiko; Scheibye-Knudsen, Morten; Neretti, Nicola; Eickelberg, Oliver; Adams, Peter D; Hu, Qianjiang; Zhu, Quan; Porritt, Rebecca A; Dong, Runze; Peters, Samuel; Victorelli, Stella; Pengo, Thomas; Khaliullin, Timur; Suryadevara, Vidyani; Fu, Xiaonan; Bar-Joseph, Ziv; Ji, Zhicheng; Passos, João F.
Afiliação
  • Gurkar AU; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Gerencser AA; Buck Institute for Research on Aging, Novato, CA, USA.
  • Mora AL; Dorothy M. Davis Heart and Lung Research Institute, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, the Ohio State University, Columbus, OH, USA.
  • Nelson AC; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Zhang AR; Division of Pulmonary, Allergy, and Critical Care Medicine, Department of Medicine and Department of Biostatistics and Bioinformatics, Duke University School of Medicine, Durham, NC, USA.
  • Lagnado AB; Department of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA.
  • Enninful A; Department of Biomedical Engineering, Yale University, New Haven, CT, USA.
  • Benz C; Buck Institute for Research on Aging, Novato, CA, USA.
  • Furman D; Buck Institute for Research on Aging, Novato, CA, USA.
  • Beaulieu D; Stanford 1000 Immunomes Project, Stanford School of Medicine, Stanford University, Stanford, CA, USA.
  • Jurk D; Instituto de Investigaciones en Medicina Traslacional (IIMT), Universidad Austral, Pilar, Argentina.
  • Thompson EL; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Wu F; Department of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA.
  • Rodriguez F; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Barthel G; Buck Institute for Research on Aging, Novato, CA, USA.
  • Chen H; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Phatnani H; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Heckenbach I; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA, USA.
  • Chuang JH; Columbia University Irving Medical Center and New York Genome Center, Columbia University, New York, NY, USA.
  • Horrell J; Buck Institute for Research on Aging, Novato, CA, USA.
  • Petrescu J; The Jackson Laboratory for Genomic Medicine, Farmington, CT, USA.
  • Alder JK; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
  • Lee JH; Columbia University Irving Medical Center and New York Genome Center, Columbia University, New York, NY, USA.
  • Niedernhofer LJ; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Kumar M; Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA.
  • Königshoff M; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Bueno M; Department of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
  • Sokka M; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Scheibye-Knudsen M; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Neretti N; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
  • Eickelberg O; Buck Institute for Research on Aging, Novato, CA, USA.
  • Adams PD; Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, RI, USA.
  • Hu Q; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Zhu Q; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.
  • Porritt RA; Aging Institute, University of Pittsburgh School of Medicine/UPMC and Division of Pulmonary, Allergy and Critical Care Medicine, Allergy, Critical Care and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, USA.
  • Dong R; University of California, San Diego, CA, USA.
  • Peters S; Sanford Burnham Prebys Medical Discovery Institute, La Jolla, CA, USA.
  • Victorelli S; Department of Biochemistry, Institute for Protein Design and Graduate Program in Biological Physics, Structure and Design, University of Washington, Seattle, WA, USA.
  • Pengo T; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Khaliullin T; Department of Physiology and Biomedical Engineering, Robert and Arlene Kogod Center on Aging, Mayo Clinic, Rochester, MN, USA.
  • Suryadevara V; Department of Laboratory Medicine and Pathology, Department of Biochemistry, Molecular Biology and Biophysics, Department of Neuroscience and Institute on the Biology of Aging and Metabolism, Minnesota Supercomputing Institute, University of Minnesota, Minneapolis, MN, USA.
  • Fu X; Dorothy M. Davis Heart and Lung Research Institute, Division of Pulmonary, Critical Care and Sleep Medicine, Department of Internal Medicine, the Ohio State University, Columbus, OH, USA.
  • Bar-Joseph Z; Department of Radiology, Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
  • Ji Z; Department of Biochemistry, Institute for Protein Design and Graduate Program in Biological Physics, Structure and Design, University of Washington, Seattle, WA, USA.
  • Passos JF; Computational Biology Department, School of Computer Science, Carnegie Mellon University, Pittsburgh, PA, USA.
Nat Aging ; 3(7): 776-790, 2023 07.
Article em En | MEDLINE | ID: mdl-37400722
ABSTRACT
Cellular senescence is a well-established driver of aging and age-related diseases. There are many challenges to mapping senescent cells in tissues such as the absence of specific markers and their relatively low abundance and vast heterogeneity. Single-cell technologies have allowed unprecedented characterization of senescence; however, many methodologies fail to provide spatial insights. The spatial component is essential, as senescent cells communicate with neighboring cells, impacting their function and the composition of extracellular space. The Cellular Senescence Network (SenNet), a National Institutes of Health (NIH) Common Fund initiative, aims to map senescent cells across the lifespan of humans and mice. Here, we provide a comprehensive review of the existing and emerging methodologies for spatial imaging and their application toward mapping senescent cells. Moreover, we discuss the limitations and challenges inherent to each technology. We argue that the development of spatially resolved methods is essential toward the goal of attaining an atlas of senescent cells.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Envelhecimento / Senescência Celular Idioma: En Ano de publicação: 2023 Tipo de documento: Article
Texto completo
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XML
PubMed Links
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Envelhecimento / Senescência Celular Idioma: En Ano de publicação: 2023 Tipo de documento: Article