Video analysis of ex vivo beating hearts during preservation on the TransMedics® organ care system.
Front Cardiovasc Med
; 10: 1216917, 2023.
Article
em En
| MEDLINE
| ID: mdl-37408655
ABSTRACT
Background:
Reliable biomarkers for assessing the viability of the donor hearts undergoing ex vivo perfusion remain elusive. A unique feature of normothermic ex vivo perfusion on the TransMedics® Organ Care System (OCS™) is that the donor heart is maintained in a beating state throughout the preservation period. We applied a video algorithm for an in vivo assessment of cardiac kinematics, video kinematic evaluation (Vi.Ki.E.), to the donor hearts undergoing ex vivo perfusion on the OCS™ to assess the feasibility of applying this algorithm in this setting.Methods:
Healthy donor porcine hearts (n = 6) were procured from Yucatan pigs and underwent 2â h of normothermic ex vivo perfusion on the OCS™ device. During the preservation period, serial high-resolution videos were captured at 30 frames per second. Using Vi.Ki.E., we assessed the force, energy, contractility, and trajectory parameters of each heart.Results:
There were no significant changes in any of the measured parameters of the heart on the OCS™ device over time as judged by linear regression analysis. Importantly, there were no significant changes in contractility during the duration of the preservation period (time 0-30â min, 918 ± 430â px/s; time 31-60â min, 1,386 ± 603â px/s; time 61-90â min, 1,299 ± 617â px/s; time 91-120â min, 1,535 ± 728â px/s). Similarly, there were no significant changes in the force, energy, or trajectory parameters. Post-transplantation echocardiograms demonstrated robust contractility of each allograft.Conclusion:
Vi.Ki.E. assessment of the donor hearts undergoing ex vivo perfusion is feasible on the TransMedics OCS™, and we observed that the donor hearts maintain steady kinematic measurements throughout the duration.
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MEDLINE
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article