Unicellular cyanobacteria degrade sulfoxaflor to its amide metabolite of potentially higher aquatic toxicity.

ABSTRACT

Sulfoxaflor (SFX) is a fourth-generation neonicotinoid used widely in modern agriculture. Due to its high water solubility and mobility in environment, it is expected to occur in water environment. Degradation of SFX leads to formation of corresponding amide (M474), which in the light of recent studies may be much more toxic to aquatic organisms than the parent molecule. Therefore, the aim of the study was to assess the potential of two common species of unicellular bloom-forming cyanobacteria (Synechocystis salina and Microcystis aeruginosa) to metabolize SFX in a 14-day-long experiment, using elevated (10 mg L-1) and predicted highest environmental (10 µg L-1) concentrations. The results obtained support the occurrence of SFX metabolism in cyanobacterial monocultures, leading to release of M474 into the water. Differential SFX decline in culture media, followed by the presence of M474, was observed for both species at different concentration levels. For S. salina, SFX concentration decreased by 7.6% at lower concentration and by 21.3% at higher concentration; the M474 concentrations were 436 ng L-1 and 514 µg L-1, respectively. Corresponding values for M. aeruginosa were 14.3% and 3.0% for SFX decline; 282 ng L-1 and 317 µg L-1 for M474 concentration. In the same time, abiotic degradation was almost non-existent. Metabolic fate of SFX was then studied for its elevated starting concentration. Uptake of SFX to cells and amounts of M474 released to water fully addressed the decrease in SFX concentration in M. aeruginosa culture, while in S. salina 15.5% of initial SFX was transformed to yet unknown metabolites. The degradation rate of SFX observed in the present study is sufficient to produce a concentration of M474 that is potentially toxic for aquatic invertebrates during cyanobacterial blooms. Therefore, there is a need for more reliable risk assessment for the presence of SFX in natural waters.