Labdane diterpenoids from Lagopsis supina and their anti-neuroinflammatory activity.
Fitoterapia
; 169: 105611, 2023 Sep.
Article
em En
| MEDLINE
| ID: mdl-37454779
ABSTRACT
In this study, ten labdane-type diterpenoids 1-10 were isolated from a methanol extract of the whole plant Lagopsis supina, including three undescribed compounds 1-3. Their structures were determined by spectroscopic data analyses such as HR-ESI-MS, 1D, and 2D NMR, as well as comparison with literature data. At the same time, the absolute configuration of five compounds 2-5 and 10 was confirmed for the first time by the single crystal X-ray diffraction method. All the compounds were isolated from L. supina for the first time. The CCK-8 assay showed that all compounds had no significant damage to BV-2 microglial cells, and then screened their inhibitory effects of nitric oxide production stimulated by lipopolysaccharide in BV-2 microglial cells. The pharmacological results showed that compound 4 greatly inhibited LPS-stimulated NO release at the concentration of 10 µM, indicating that it has potential anti-neuroinflammatory activity.
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Base de dados:
MEDLINE
Assunto principal:
Medicamentos de Ervas Chinesas
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Lamiaceae
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Diterpenos
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article