Methods to Analyze Nutritional and Inter-Organ Control of Drosophila Ovarian Germline Stem Cells.
Methods Mol Biol
; 2677: 81-97, 2023.
Article
em En
| MEDLINE
| ID: mdl-37464236
ABSTRACT
Physiological status, particularly dietary input, has major impacts on the Drosophila melanogaster ovarian germline stem cell lineage. Moreover, several studies have shed light on the role that inter-organ communication plays in coordinating whole-organism responses to changes in physiology. For example, nutrient-sensing signaling pathways function within the fat body to regulate germline stem cells and their progeny in the ovary. Together with its incredible genetic and cell biological toolkits, Drosophila serves as an amenable model organism to use for uncovering molecular mechanisms that underlie physiological control of adult stem cells. In this methods chapter, we describe a general dietary manipulation paradigm, genetic manipulation of adult adipocytes, and whole-mount ovary immunofluorescence to investigate physiological control of germline stem cells.
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MEDLINE
Assunto principal:
Proteínas de Drosophila
/
Células-Tronco de Oogônios
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article