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Host hydrocarbons protect symbiont transmission from a radical host defense.
Ingham, Chantal Selina; Engl, Tobias; Matarrita-Carranza, Bernal; Vogler, Paul; Huettel, Bruno; Wielsch, Natalie; Svatos, Ales; Kaltenpoth, Martin.
Afiliação
  • Ingham CS; Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg-University Mainz, Mainz 55128, Germany.
  • Engl T; Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg-University Mainz, Mainz 55128, Germany.
  • Matarrita-Carranza B; Department of Insect Symbiosis, Max-Planck-Institute for Chemical Ecology, Jena 07745, Germany.
  • Vogler P; Department of Insect Symbiosis, Max-Planck-Institute for Chemical Ecology, Jena 07745, Germany.
  • Huettel B; Department of Evolutionary Ecology, Institute of Organismic and Molecular Evolution, Johannes Gutenberg-University Mainz, Mainz 55128, Germany.
  • Wielsch N; Max Planck Genome Centre Cologne, Max Planck Institute for Plant Breeding Research, Cologne 50829, Germany.
  • Svatos A; Research Group Mass Spectrometry/Proteomics, Max-Planck-Institute for Chemical Ecology, Jena 07745, Germany.
  • Kaltenpoth M; Research Group Mass Spectrometry/Proteomics, Max-Planck-Institute for Chemical Ecology, Jena 07745, Germany.
Proc Natl Acad Sci U S A ; 120(31): e2302721120, 2023 08.
Article em En | MEDLINE | ID: mdl-37487102
Symbioses with microbes play a pivotal role in the evolutionary success of insects, and can lead to intimate host-symbiont associations. However, how the host maintains a stable symbiosis with its beneficial partners while keeping antagonistic microbes in check remains incompletely understood. Here, we uncover a mechanism by which a host protects its symbiont from the host's own broad-range antimicrobial defense during transmission. Beewolves, a group of solitary digger wasps (Hymenoptera: Crabronidae), provide their brood cells with symbiotic Streptomyces bacteria that are later transferred to the cocoon and protect the offspring from opportunistic pathogens by producing antibiotics. In the brood cell, however, the symbiont-containing secretion is exposed to a toxic burst of nitric oxide (NO) released by the beewolf egg, which effectively kills antagonistic microorganisms. How the symbiont survives this lethal NO burst remained unknown. Here, we report that upon NO exposure in vitro, the symbionts mount a global stress response, but this is insufficient to ensure survival at brood cell-level NO concentrations. Instead, in vivo bioassays demonstrate that the host's antennal gland secretion (AGS) surrounding the symbionts in the brood cell provides an effective diffusion barrier against NO. This physicochemical protection can be reconstituted in vitro by beewolf hydrocarbon extracts and synthetic hydrocarbons, indicating that the host-derived long-chain alkenes and alkanes in the AGS are responsible for shielding the symbionts from NO. Our results reveal how host adaptations can protect a symbiont from host-generated oxidative and nitrosative stress during transmission, thereby efficiently balancing pathogen defense and mutualism maintenance.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Himenópteros / Anti-Infecciosos Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Himenópteros / Anti-Infecciosos Idioma: En Ano de publicação: 2023 Tipo de documento: Article