Metal-Organic Framework as a Fluorescent and Colorimetric Dual-Signal Readout Biosensor Platform for the Detection of a Genetic Sequence from the SARS-CoV-2 Genome.
ACS Appl Mater Interfaces
; 15(32): 38163-38170, 2023 Aug 16.
Article
em En
| MEDLINE
| ID: mdl-37535905
ABSTRACT
The quest for the development of high-accuracy, point-of-care, and cost-effective testing platforms for SARS-CoV-2 infections is ongoing as current diagnostics rely on either assays based on costly yet accurate nucleic acid amplification tests (NAAT) or less selective and less sensitive but rapid and cost-effective antigen tests. As a potential solution, this work presents a fluorescence-based detection platform using a metal-organic framework (MOF) in an effective assay, demonstrating the potential of MOFs to recognize specific targets of the SARS-CoV-2 genome with high accuracy and rapid process turnaround time. As a highlight of this work, positive detection of SARS-CoV-2 is indicated by a visible color change of the MOF probe with ultrahigh detection selectivities down to single-base mismatch nucleotide sequences, thereby providing an alternative avenue for the development of innovative detection methods for diverse viral genomes.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Estruturas Metalorgânicas
/
COVID-19
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article