Female naïve human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations.
Sci Adv
; 9(31): eadf2245, 2023 08 04.
Article
em En
| MEDLINE
| ID: mdl-37540754
ABSTRACT
Three-dimensional (3D) genomics shows immense promise for studying X chromosome inactivation (XCI) by interrogating changes to the X chromosomes' 3D states. Here, we sought to characterize the 3D state of the X chromosome in naïve and primed human pluripotent stem cells (hPSCs). Using chromatin tracing, we analyzed X chromosome folding conformations in these cells with megabase genomic resolution. X chromosomes in female naïve hPSCs exhibit folding conformations similar to the active X chromosome (Xa) and the inactive X chromosome (Xi) in somatic cells. However, naïve X chromosomes do not exhibit the chromatin compaction typically associated with these somatic X chromosome states. In H7 naïve human embryonic stem cells, XIST accumulation observed on damaged X chromosomes demonstrates the potential for naïve hPSCs to activate XCI-related mechanisms. Overall, our findings provide insight into the X chromosome status of naïve hPSCs with a single-chromosome resolution and are critical in understanding the unique epigenetic regulation in early embryonic cells.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Pluripotentes
/
RNA Longo não Codificante
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article