Droplet-based single-cell joint profiling of histone modifications and transcriptomes.
Nat Struct Mol Biol
; 30(10): 1428-1433, 2023 10.
Article
em En
| MEDLINE
| ID: mdl-37563440
ABSTRACT
We previously reported Paired-Tag, a combinatorial indexing-based method that can simultaneously map histone modifications and gene expression at single-cell resolution at scale. However, the lengthy procedure of Paired-Tag has hindered its general adoption in the community. To address this bottleneck, we developed a droplet-based Paired-Tag protocol that is faster and more accessible than the previous method. Using cultured mammalian cells and primary brain tissues, we demonstrate its superior performance at identifying candidate cis-regulatory elements and associating their dynamic chromatin state to target gene expression in each constituent cell type in a complex tissue.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Código das Histonas
/
Transcriptoma
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article