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Direct differentiation of human pluripotent stem cells into vascular network along with supporting mural cells.
Bertucci, Taylor; Kakarla, Shravani; Winkelman, Max A; Lane, Keith; Stevens, Katherine; Lotz, Steven; Grath, Alexander; James, Daylon; Temple, Sally; Dai, Guohao.
Afiliação
  • Bertucci T; Neural Stem Cell Institute, Rensselaer, New York 12144, USA.
  • Kakarla S; Northeastern University, Department of Bioengineering, Boston, Massachusetts 02115, USA.
  • Winkelman MA; Northeastern University, Department of Bioengineering, Boston, Massachusetts 02115, USA.
  • Lane K; Neural Stem Cell Institute, Rensselaer, New York 12144, USA.
  • Stevens K; Neural Stem Cell Institute, Rensselaer, New York 12144, USA.
  • Lotz S; Neural Stem Cell Institute, Rensselaer, New York 12144, USA.
  • Grath A; Northeastern University, Department of Bioengineering, Boston, Massachusetts 02115, USA.
  • James D; Weill Cornell Medicine, New York, New York 10065, USA.
  • Temple S; Neural Stem Cell Institute, Rensselaer, New York 12144, USA.
  • Dai G; Northeastern University, Department of Bioengineering, Boston, Massachusetts 02115, USA.
APL Bioeng ; 7(3): 036107, 2023 Sep.
Article em En | MEDLINE | ID: mdl-37564277
During embryonic development, endothelial cells (ECs) undergo vasculogenesis to form a primitive plexus and assemble into networks comprised of mural cell-stabilized vessels with molecularly distinct artery and vein signatures. This organized vasculature is established prior to the initiation of blood flow and depends on a sequence of complex signaling events elucidated primarily in animal models, but less studied and understood in humans. Here, we have developed a simple vascular differentiation protocol for human pluripotent stem cells that generates ECs, pericytes, and smooth muscle cells simultaneously. When this protocol is applied in a 3D hydrogel, we demonstrate that it recapitulates the dynamic processes of early human vessel formation, including acquisition of distinct arterial and venous fates, resulting in a vasculogenesis angiogenesis model plexus (VAMP). The VAMP captures the major stages of vasculogenesis, angiogenesis, and vascular network formation and is a simple, rapid, scalable model system for studying early human vascular development in vitro.

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Idioma: En Ano de publicação: 2023 Tipo de documento: Article