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Antidiabetic effects of polyherbal mixture made of Centaurium erythraea, Cichorium intybus and Potentilla erecta.
Petrovic, Aleksandra; Madic, Visnja; Stojanovic, Gordana; Zlatanovic, Ivana; Zlatkovic, Bojan; Vasiljevic, Perica; Dordevic, Ljubisa.
Afiliação
  • Petrovic A; Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia. Electronic address: aleksandra.petrovic2@pmf.edu.rs.
  • Madic V; Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia.
  • Stojanovic G; Department of Chemistry, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia.
  • Zlatanovic I; Department of Chemistry, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia.
  • Zlatkovic B; Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia.
  • Vasiljevic P; Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia.
  • Dordevic L; Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Nis, Visegradska 33, 18000, Nis, Serbia.
J Ethnopharmacol ; 319(Pt 1): 117032, 2024 Jan 30.
Article em En | MEDLINE | ID: mdl-37582477
ABSTRACT
ETHNOPHARMACOLOGICAL RELEVANCE The polyherbal mixture made of Centaurium erythraea aerial parts and Cichorium intybus roots and Potentilla erecta rhizomes has been used for centuries to treat both the primary and secondary complications of diabetes. AIM OF THE STUDY As a continuation of our search for the most effective herbal mixture used as an ethnopharmacological remedy for diabetes, this study aimed to compare the in vitro biological activities of this polyherbal mixture and its individual ingredients, and, most importantly, to validate the ethnopharmacological value of the herbal mixture through evaluation of its phytochemical composition, its potential in vivo toxicity and its effect on diabetes complications. MATERIALS AND

METHODS:

Phytochemical analysis was performed using HPLC-UV. Antioxidant activity was estimated via the DPPH test. Potential cytotoxicity/anticytotoxicity was assessed using an in vitro RBCs antihemolytic assay and an in vivo sub-chronic oral toxicity method. Antidiabetic activity was evaluated using an in vitro α-amylase inhibition assay and in vivo using a chemically induced diabetic rat model.

RESULTS:

The HPLC-UV analysis revealed the presence of p-hydroxybenzoic acid, p-hydroxybenzoic acid derivative, catechin, five catechin derivatives, epicatechin, isoquercetin, hyperoside, rutin, four quercetin derivatives, caffeic acid, and four caffeic acid derivatives in the polyherbal mixture decoction. Treatment with the decoction has shown no toxic effects. The antioxidant and cytoprotective activities of the polyherbal mixture were higher than the reference's ones. Its antidiabetic activity was high in both in vitro and in vivo studies. Fourteen days of treatment with the decoction (15 g/kg) completely normalized blood glucose levels of diabetic animals, while treatments with insulin and glimepiride only slightly lowered glycemic values. In addition, lipid status of treated animals as well as levels of serum AST, ALT, ALP, creatinine, urea and MDA were completely normalized. In addition, the polyherbal mixture completely restored the histopathological changes of the liver, kidneys and all four Cornu ammonis regions of the hippocampus.

CONCLUSIONS:

The polyherbal mixture was effective in the prevention of both primary and secondary diabetic complications such as hyperlipidemia, increased lipid peroxidation, non-alcoholic fatty liver disease, nephropathy and neurodegeneration.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Catequina / Cichorium intybus / Centaurium / Potentilla / Diabetes Mellitus Experimental Idioma: En Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Catequina / Cichorium intybus / Centaurium / Potentilla / Diabetes Mellitus Experimental Idioma: En Ano de publicação: 2024 Tipo de documento: Article