Rapid RGR-dependent visual pigment recycling is mediated by the RPE and specialized Müller glia.
Cell Rep
; 42(8): 112982, 2023 08 29.
Article
em En
| MEDLINE
| ID: mdl-37585292
ABSTRACT
In daylight, demand for visual chromophore (11-cis-retinal) exceeds supply by the classical visual cycle. This shortfall is compensated, in part, by the retinal G-protein-coupled receptor (RGR) photoisomerase, which is expressed in both the retinal pigment epithelium (RPE) and in Müller cells. The relative contributions of these two cellular pools of RGR to the maintenance of photoreceptor light responses are not known. Here, we use a cell-specific gene reactivation approach to elucidate the kinetics of RGR-mediated recovery of photoreceptor responses following light exposure. Electroretinographic measurements in mice with RGR expression limited to either cell type reveal that the RPE and a specialized subset of Müller glia contribute both to scotopic and photopic function. We demonstrate that 11-cis-retinal formed through photoisomerization is rapidly hydrolyzed, consistent with its role in a rapid visual pigment regeneration process. Our study shows that RGR provides a pan-retinal sink for all-trans-retinal released under sustained light conditions and supports rapid chromophore regeneration through the photic visual cycle.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Retinaldeído
/
Epitélio Pigmentado da Retina
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article