Supplementary sulfide during inoculation for improved sulfur autotrophic denitrification performance and adaptation to low temperature.

Elemental sulfur (S0) autotrophic denitrification (SAD) has been considered an advanced denitrification technology due to its low operating cost and small secondary pollution in wastewater treatment plants. However, the wide application of this technology is still challenged by its low denitrification rate, long start-up time, and poor low-temperature adaptation. This study employed supplementary sulfide to facilitate the conversion of S0 into polysulfide, a critical step in SAD. Batch experiments indicated that more polysulfide could be generated when S0 served as an electron donor and partnered with additional Na2S, leading to greatly increased nitrate removal than the controls. Particularly when the sulfide concentration was relatively high at 160 mg/L, a denitrification rate up to 11.3 mg-N/(L·d) was achieved, 3.8-fold of control group working with solely S0. Sulfide was further applied during inoculation of a packed bed reactor (PBR) with S0 particles and significantly benefit the development of biofilm. Although the feeding of sulfide was stopped after inoculation, the reactor was fast started up in just 2 days and delivered an average denitrification rate of 346.9 mg-N/(L·d), 1.4-fold of the control. In addition, benefit from the thick and well-developed biofilm, the reactor was able to restore its nitrate removal performance, when challenged by a low temperature (15 °C), to a larger rate than the control. Compared to short-term employment of the sulfide which was found a temporary solution addressing declined SAD rate during operating the PBR, applying sulfide for inoculation facilitated the formation of biofilm, leading to sustained improvement of SAD performance and better adaptation to coldness.