Enhancement of cell migration and wound healing by nano-herb ointment formulated with biosurfactant, silver nanoparticles and Tridax procumbens.
Front Microbiol
; 14: 1225769, 2023.
Article
em En
| MEDLINE
| ID: mdl-37601383
ABSTRACT
Introduction:
Ointments are generally used as a therapeutic agent for topical medication or transdermal drug delivery, such as wound healing and skin lesions.Methods:
In this study, Tridax procumbens plant extract (0.7 g/mL) was used to prepare herbal-infused oil as the oil phase and gelatin-stabilized silver nanoparticle (G-AgNPs) (0.3 g/mL) as the aqueous phase. To blend the oil and aqueous phases, rhamnolipid biosurfactant with a critical micelle concentration of 55 mg/L from strain Pseudomonas aeruginosa PP4 has been used for herb ointment preparation. The average size of the synthesized G-AgNPs was observed between 10-30 nm and confirmed as spherical-shaped particles by TEM analysis. Subsequently, GC-MS and FTIR characterization are used to confirm herb ointment's chemical and functional characteristics.Results:
Based on the antibacterial studies, the highest microbial growth inhibition was observed for herb ointment, about 19.5 mm for the pathogen Staphylococcus aureus at the concentration of 100 µg/mL, whereas 15.5 mm was obtained for Escherichia coli, respectively. In addition, the minimum inhibitory concentration (MIC) assay showed negligible bacterial growth at 100 µg/mL for S. aureus and E. coli, respectively. Moreover, the cell viability assay for herb ointment exhibited low cytotoxic activity at higher concentrations (100 µg/mL) in Vero cell lines. In this study, wound scratch assay showed a significant cell migration rate (90 ± 2%) in 3 days of incubation than the control (62 ± 2%).Discussion:
As a result, the biosurfactant-based nano-topical herb ointment revealed a low cytotoxic and higher cell migration capacity. Altogether, these findings highlighted the utility of this herb ointment in therapeutic applications such as wound healing.
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Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article