[Electroacupuncture at "Baihui"ï¼GV20ï¼ and "Yongquan"ï¼KI1ï¼ improves learning-memory ability of APP/PS1 double transgenic mice by regulating endosomal-lysosomal system].
Zhen Ci Yan Jiu
; 48(8): 791-8, 2023 Aug 25.
Article
em Zh
| MEDLINE
| ID: mdl-37614137
ABSTRACT
OBJECTIVE:
To explore the mechanism of electroacupunctureï¼EAï¼ in improving learning-memory ability in Alzheimer's disease ï¼ADï¼ mice from the perspective of endosomal-lysosomal system.METHODS:
Male APP/PS1 transgenic mice were randomly divided into model group and EA group ï¼n=10 in each groupï¼ and 10 male C57BL/6 wild mice were taken as the normal group. EA ï¼1 Hz/50 Hz, 1 mAï¼ was applied at bilateral "Yongquan"ï¼KI1ï¼ and acupuncture was applied at "Baihui" ï¼GV20ï¼ for 15 min. The mice of the model and normal groups were subjected to restriction with the same method as those of the EA group for 15 min. The treatment was conducted once every other day for 6 weeks. The spatial learning-memory ability ï¼shown by escape latency of place navigation test and the time of crossing the target platform and total swimming distance in the target quadrant in 1 min of spatial probe test ï¼ was detected by Morris water maze test. The immunoactivity of senile plaques ï¼SPï¼ in the hippocampus tissue was detected by immunohistochemistry. The ultrastructural characters of hippocampal neurons were observed by transmission electron microscope, and the expression levels of Ras-related protein 5 ï¼Rab5ï¼, Ras-related protein 7 ï¼Rab7ï¼ and cathepsin D ï¼CTSDï¼ in the hippocampus were detected by Western blot, separately.RESULTS:
Compared with the normal group, the escape latency, SP immunoactivity, and protein expression levels of Rab5, Rab7 and CTSD were significantly increased ï¼P<0.05, P<0.01ï¼, while the number of crossing the original platform and the total swimming distance in the platform quadrant were considerably reduced ï¼P<0.05ï¼ in the model group. In contrast to the model group, the EA group had a marked decrease in the escape latency, SP immunoactivity, and protein expression levels of Rab5, Rab7 and CTSD ï¼P<0.05, P<0.01ï¼, and a striking increase in the number of crossing the original platform and the swimming distance in the platform quadrant ï¼P<0.05ï¼. Results of transmission electron microscope showed an accumulation of endosome, lysosome, and endolysosomes in the hippocampal neurons in the model group, which was evidently milder in the EA group.CONCLUSION:
EA of GV20 and KI1 can improve the learning-memory ability of AD mice, which may be related to its function in reducing hippocampal Aß deposition and down-regulating endosomal-lysosomal system activity.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Eletroacupuntura
/
Doença de Alzheimer
Idioma:
Zh
Ano de publicação:
2023
Tipo de documento:
Article