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Paper-Based Biosensor for the Detection of Sepsis Using MMP-9 Biomarker in FIP Mice Model.
Alekhmimi, Nuha Khalid; Raddadi, Zeyad; Alabdulwahed, Abdulelah A; Eissa, Shimaa; Cialla-May, Dana; Popp, Jürgen; Al-Kattan, Khaled; Zourob, Mohammed.
Afiliação
  • Alekhmimi NK; Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, AlTakhassusi Rd, Riyadh 11533, Saudi Arabia.
  • Raddadi Z; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, 07745 Jena, Germany.
  • Alabdulwahed AA; Cell Therapy and Immunobiology Department, King Faisal Specialist Hospital and Research Center, Riyadh 13541, Saudi Arabia.
  • Eissa S; Saudi Food and Drug Authority, Riyadh 13513, Saudi Arabia.
  • Cialla-May D; Department of Chemistry, Khalifa University of Science and Technology, Abu Dhabi P.O. Box 127788, United Arab Emirates.
  • Popp J; Advanced Materials Chemistry Center (AMCC), Khalifa University of Science and Technology, Abu Dhabi P.O. Box 127788, United Arab Emirates.
  • Al-Kattan K; Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University, 07745 Jena, Germany.
  • Zourob M; Leibniz Institute of Photonic Technology, Member of Leibniz Health Technologies, Member of the Leibniz Centre for Photonics in Infection Research (LPI), Albert Einstein Straße 9, 07745 Jena, Germany.
Biosensors (Basel) ; 13(8)2023 Aug 11.
Article em En | MEDLINE | ID: mdl-37622890
ABSTRACT
Sepsis is an immune response to a microbial invasion that causes organ injury and dysfunction due to a systemic inflammatory response. Sepsis is a serious, life-threatening condition and a widely recognized global health challenge. Given its high death rate, it is critical to diagnose sepsis and start treatment as early as possible. There is an urgent need for a sensitive and rapid screening method for detecting sepsis. In this study, we investigated the use of MMP-9 as a biomarker for sepsis. A colorimetric paper-based biosensor was used for the detection of MMP-9 utilizing peptide-magnetic nanoparticle conjugates. The method is based on the cleavage of the MMP-9-specific peptide by the protease leading to the detaching of the magnetic beads from the sensor surface and changing of color. A fecal intraperitoneal (FIP) challenge was used to induce sepsis in mice, and an MMP-9 secretion was measured by taking blood and Bronchoalveolar Lavage (BAL) fluid samples at 1 h, 2 h, 4 h, and 20 h (early sepsis) post-challenge intervals. The results of the paper-based sensor for the detection of MMP-9 levels in blood samples and BAL samples were compared with ELISA and Western Blot. We found that both blood and BAL levels of MMP-9 increased immediately and could be detected as early as 1 h in FIP mice post-challenge. Our work adds evidence to the assertion that MMP-9 is a reliable biomarker for the detection of sepsis at early stages.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sepse / Metaloproteinase 9 da Matriz Idioma: En Ano de publicação: 2023 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sepse / Metaloproteinase 9 da Matriz Idioma: En Ano de publicação: 2023 Tipo de documento: Article