Dexpanthenol improved stem cells against cisplatin-induced kidney injury by inhibition of TNF-α, TGFß-1, ß-catenin, and fibronectin pathways.
Saudi J Biol Sci
; 30(9): 103773, 2023 Sep.
Article
em En
| MEDLINE
| ID: mdl-37635837
ABSTRACT
Introduction:
Cisplatin interacts with DNA and induces an immunological response and reactive oxygen species, which are nephrotoxic mediators. Stem cells self-renew through symmetric divisions and can develop into other cell types due to their multipotency. Dexpanthenol has been proven to protect against renal injury.Aim:
This study aims to demonstrate that dexpanthenol could improve the effect of adipose-derived mesenchymal stem cells (ADMSC) against cisplatin-induced acute kidney injury.Methods:
Sixty male Sprague-Dawley rats were divided into 5 groups (N = 12) control, cisplatin, cisplatin & dexpanthenol, cisplatin & ADMSC, and cisplatin & dexpanthenol & ADMSCs. On the 5th day following cisplatin injection, half the rats in each group were sacrificed, and the other half were sacrificed on the 12th day. Histopathological examination, molecular studies (IL-6, Bcl2, TGFß-1, Caspase-3, Fibronectin, and ß-catenin), antioxidants (superoxide dismutase and catalase), and renal function were all investigated.Results:
In contrast to cisplatin group, the dexpanthenol and ADMSCs treatments significantly decreased renal function and oxidative stress while significantly enhancing antioxidants. Dexpanthenol improved stem cells by significantly down-regulating caspase-3, IL-6, TGF-ß1, Fibronectin, and ß-catenin and significantly up-regulating Bcl2 and CD34, which reversed the cisplatin effect.Conclusion:
Dexpanthenol enhanced ADMSCs' ability to protect against cisplatin-induced AKI by decreasing inflammation, apoptosis, and fibrosis.
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MEDLINE
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En
Ano de publicação:
2023
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Article