Reverse vaccinology and immunoinformatics approaches to design multi-epitope based vaccine against oncogenic KRAS.
Med Oncol
; 40(10): 283, 2023 Aug 29.
Article
em En
| MEDLINE
| ID: mdl-37644143
ABSTRACT
Mutant KRAS-induced tumorigenesis is highly involved in the progression of pancreatic, lung, and breast cancer. Comparatively, KRAS G12D and KRAS G12C are the most frequent mutations that promote cancer progression and aggressiveness. Although KRAS mutant inhibitors exhibit significant therapeutic potential, day by day, they are becoming resistant among patients. Multi-epitope based cancer vaccines are a promising alternative strategy that induces an immune response against tumor antigens. In the present study, we have designed, constructed, and validated a novel multi-epitope vaccine construct against KRAS G12D and G12C mutants using reverse vaccinology and immunoinformatics approaches. In addition, the vaccine construct was structurally refined and showed significant physiochemical properties, and could induce an immune response. Furthermore, the optimized vaccine construct was cloned into a pET28a (+) expression vector through in silico cloning. Conclusively, the multi-epitope vaccine construct is structurally stable, soluble, antigenic, nonallergic, and nontoxic. Further, it has to be studied in in vitro and in vivo to evaluate its therapeutic efficacy against KRAS-mutated cancers in the near future.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias da Mama
/
Vacinas Anticâncer
Idioma:
En
Ano de publicação:
2023
Tipo de documento:
Article